Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides

Eikan Mishima; Daisuke Jinno; Yasutoshi Akiyama; Kunihiko Itoh; Shinnosuke Nankumo; Hisato Shima; Koichi Kikuchi; Yoichi Takeuchi; Alaa Elkordy; Takehiro Suzuki; Kuniyasu Niizuma; Sadayoshi Ito; Yoshihisa Tomioka; Takaaki Abe

doi:10.1371/journal.pone.0143756

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Peer-reviewed

Research Article

Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides

Eikan Mishima ,

Contributed equally to this work with: Eikan Mishima, Daisuke Jinno

Affiliations Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan, Tohoku Medical Megabank Organization, Sendai, Japan
⨯
Daisuke Jinno ,

Contributed equally to this work with: Eikan Mishima, Daisuke Jinno

Affiliation Laboratory of Oncology, Pharmacy Practice and Sciences, Tohoku University Graduate School of Pharmaceutical Sciences, Sendai, Japan
⨯
Yasutoshi Akiyama,

Affiliations Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan, Tohoku Medical Megabank Organization, Sendai, Japan
⨯
Kunihiko Itoh,

Affiliation Department of Clinical Pharmacology and Genetics, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan
⨯
Shinnosuke Nankumo,

Affiliation Laboratory of Oncology, Pharmacy Practice and Sciences, Tohoku University Graduate School of Pharmaceutical Sciences, Sendai, Japan
⨯
Hisato Shima,

Affiliation Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Koichi Kikuchi,

Affiliation Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Yoichi Takeuchi,

Affiliation Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Alaa Elkordy,

Affiliation Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Takehiro Suzuki,

Affiliation Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Kuniyasu Niizuma,

Affiliation Department of Neurosurgery, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Sadayoshi Ito,

Affiliation Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯
Yoshihisa Tomioka,

Affiliation Laboratory of Oncology, Pharmacy Practice and Sciences, Tohoku University Graduate School of Pharmaceutical Sciences, Sendai, Japan
⨯
Takaaki Abe

* E-mail: takaabe@med.tohoku.ac.jp

Affiliations Division of Nephrology, Endocrinology, and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan, Department of Medical Science, Tohoku University Graduate School of Biomedical Engineering, Sendai, Japan, Department of Clinical Biology and Hormonal Regulation, Tohoku University Graduate School of Medicine, Sendai, Japan
⨯

Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides

Eikan Mishima,
Daisuke Jinno,
Yasutoshi Akiyama,
Kunihiko Itoh,
Shinnosuke Nankumo,
Hisato Shima,
Koichi Kikuchi,
Yoichi Takeuchi,
Alaa Elkordy,
Takehiro Suzuki

Published: November 25, 2015
https://doi.org/10.1371/journal.pone.0143756

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