Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses

Jayme Parker; Nisha Fowler; Mary Louise Walmsley; Terri Schmidt; Jason Scharrer; James Kowaleski; Teresa Grimes; Shanann Hoyos; Jack Chen

doi:10.1371/journal.pone.0143164

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Open Access

Peer-reviewed

Research Article

Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses

Jayme Parker,

Affiliations Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America, Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America
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Nisha Fowler,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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Mary Louise Walmsley,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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Terri Schmidt,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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Jason Scharrer,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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James Kowaleski,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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Teresa Grimes,

Affiliation Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America
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Shanann Hoyos,

Affiliation Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America
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Jack Chen

* E-mail: j.chen@alaska.edu

Affiliations Department of Health and Social Services, Division of Public Health, Alaska State Public Health Virology Laboratory, Fairbanks, Alaska, United States of America, Department of Biology and Wildlife, Institute of Arctic Biology, University of Alaska Fairbanks, Fairbanks, Alaska, United States of America
⨯

Analytical Sensitivity Comparison between Singleplex Real-Time PCR and a Multiplex PCR Platform for Detecting Respiratory Viruses

Jayme Parker,
Nisha Fowler,
Mary Louise Walmsley,
Terri Schmidt,
Jason Scharrer,
James Kowaleski,
Teresa Grimes,
Shanann Hoyos,
Jack Chen

Published: November 16, 2015
https://doi.org/10.1371/journal.pone.0143164

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