A Versatile Strategy for Production of Membrane Proteins with Diverse Topologies: Application to Investigation of Bacterial Homologues of Human Divalent Metal Ion and Nucleoside Transporters

• Cheng Ma ,

  Contributed equally to this work with: Cheng Ma, Zhenyu Hao

  Current address: Institute of Molecular & Experimental Medicine, Wales Heart Research Institute, Academic Avenue, Heath Park, Cardiff, CF14 4XN, United Kingdom

  Affiliation Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom

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• Zhenyu Hao ,

  Contributed equally to this work with: Cheng Ma, Zhenyu Hao

  Affiliations Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin, China, Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom

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• Gerard Huysmans,

  Current address: Weill Cornell Medical College Whitney Pavilion, 1300 York Avenue New York, New York, 10065, United States of America

  Affiliation Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom

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• Amelia Lesiuk,

  Affiliation Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom

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• Per Bullough,

  Affiliation Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield, United Kingdom

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• Yingying Wang,

  Affiliation Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), College of Environmental Science and Engineering, Nankai University, Tianjin, China

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• Mark Bartlam,

  Affiliations Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom, College of Life Sciences, Nankai University, Tianjin, China, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin, China

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• Simon E. Phillips,

  Affiliation Research Complex at Harwell, Harwell Science and Innovation Campus, Didcot, Oxfordshire, United Kingdom

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• James D. Young,

  Affiliation Department of Physiology, University of Alberta, Edmonton, Canada

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• Adrian Goldman,

  Affiliations Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom, College of Life Sciences, Nankai University, Tianjin, China, Division of Biochemistry, Department of Biosciences, University of Helsinki, Helsinki, Finland

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• Stephen A. Baldwin †,

  † Deceased.

  Affiliation Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom

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• Vincent L. G. Postis

  * E-mail: v.l.g.postis@leeds.ac.uk

  Affiliations Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences, University of Leeds, Leeds, United Kingdom, Biomedicine Research Group, Faculty of Health and Social Sciences, Leeds Beckett University, Leeds, LS1 3HE, United Kingdom

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