Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based Assays

Giada Mattiuzzo; James Ashall; Kathryn S. Doris; Kirsty MacLellan-Gibson; Carolyn Nicolson; Dianna E. Wilkinson; Ruth Harvey; Neil Almond; Robert Anderson; Stacey Efstathiou; Philip D. Minor; Mark Page

doi:10.1371/journal.pone.0142751

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Open Access

Peer-reviewed

Research Article

Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based Assays

Giada Mattiuzzo ,

* E-mail: Giada.mattiuzzo@nibsc.org

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
James Ashall,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Kathryn S. Doris,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Kirsty MacLellan-Gibson,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Carolyn Nicolson,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Dianna E. Wilkinson,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Ruth Harvey,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Neil Almond,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Robert Anderson,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Stacey Efstathiou,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Philip D. Minor,

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯
Mark Page

Affiliation National Institute for Biological Standards and Controls-MHRA, South Mimms-Potters Bar, United Kingdom
⨯

Development of Lentivirus-Based Reference Materials for Ebola Virus Nucleic Acid Amplification Technology-Based Assays

Giada Mattiuzzo,
James Ashall,
Kathryn S. Doris,
Kirsty MacLellan-Gibson,
Carolyn Nicolson,
Dianna E. Wilkinson,
Ruth Harvey,
Neil Almond,
Robert Anderson,
Stacey Efstathiou

Published: November 12, 2015
https://doi.org/10.1371/journal.pone.0142751

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Ebola virus
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HIV-1
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Gene expression
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RNA extraction
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Polymerase chain reaction
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RNA stability
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Reverse transcriptase-polymerase chain reaction
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HIV
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