A Novel High-Content Immunofluorescence Assay as a Tool to Identify at the Single Cell Level γ-Globin Inducing Compounds

Marta Durlak; Cristina Fugazza; Sudharshan Elangovan; Maria Giuseppina Marini; Maria Franca Marongiu; Paolo Moi; Ivan Fraietta; Paolo Cappella; Gloria Barbarani; Isaura Font-Monclus; Mario Mauri; Sergio Ottolenghi; Fabio Gasparri; Antonella Ronchi

doi:10.1371/journal.pone.0141083

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Open Access

Peer-reviewed

Research Article

A Novel High-Content Immunofluorescence Assay as a Tool to Identify at the Single Cell Level γ-Globin Inducing Compounds

Marta Durlak,

Affiliation Department of Biology, Nerviano Medical Sciences S.r.l., Nerviano, Milano, Italy
⨯
Cristina Fugazza,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯
Sudharshan Elangovan,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯
Maria Giuseppina Marini,

Affiliation Istituto di Ricerca Genetica e Biomedica del Consiglio Nazionale delle Ricerche, Cagliari, Italy
⨯
Maria Franca Marongiu,

Affiliation Istituto di Ricerca Genetica e Biomedica del Consiglio Nazionale delle Ricerche, Cagliari, Italy
⨯
Paolo Moi,

Affiliation Dipartimento di Sanità Pubblica, Medicina Clinica e Molecolare, Università degli Studi di Cagliari, Cagliari, Italy
⨯
Ivan Fraietta,

Affiliation Department of Biology, Nerviano Medical Sciences S.r.l., Nerviano, Milano, Italy
⨯
Paolo Cappella,

Current Address: FlowMetric Europe srl, Parco Tecnologico Padano, Lodi, Italy

Affiliation Department of Biology, Nerviano Medical Sciences S.r.l., Nerviano, Milano, Italy
⨯
Gloria Barbarani,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯
Isaura Font-Monclus,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯
Mario Mauri,

Affiliation Dipartimento di Scienze della Salute, Università degli studi di Milano-Bicocca, Monza, Italy
⨯
Sergio Ottolenghi,

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯
Fabio Gasparri,

Affiliation Department of Biology, Nerviano Medical Sciences S.r.l., Nerviano, Milano, Italy
⨯
Antonella Ronchi

* E-mail: antonella.ronchi@unimib.it

Affiliation Dipartimento di Biotecnologie e Bioscienze, Università degli studi di Milano-Bicocca, Milano, Italy
⨯

A Novel High-Content Immunofluorescence Assay as a Tool to Identify at the Single Cell Level γ-Globin Inducing Compounds

Marta Durlak,
Cristina Fugazza,
Sudharshan Elangovan,
Maria Giuseppina Marini,
Maria Franca Marongiu,
Paolo Moi,
Ivan Fraietta,
Paolo Cappella,
Gloria Barbarani,
Isaura Font-Monclus

Published: October 28, 2015
https://doi.org/10.1371/journal.pone.0141083

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Globins
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Small interfering RNA
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Transfection
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Immunofluorescence
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