RNA Amplification Protocol Leads to Biased Polymerase Chain Reaction Results Especially for Low-Copy Transcripts of Human Bone Marrow-Derived Stromal Cells

Open Access

Peer-reviewed

Research Article

Carolin Coenen ,

* E-mail: Carolin.Coenen@med.uni-duesseldorf.de

Affiliation Institute of Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Medical Center, 40225, Düsseldorf, Germany
⨯
Stefanie Liedtke,

Affiliation Institute of Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Medical Center, 40225, Düsseldorf, Germany
⨯
Gesine Kogler

Affiliation Institute of Transplantation Diagnostics and Cell Therapeutics, Heinrich-Heine-University Medical Center, 40225, Düsseldorf, Germany
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Polymerase chain reaction
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Gene expression
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Reverse transcriptase-polymerase chain reaction
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RNA amplification
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Transcriptome analysis
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cDNA synthesis
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Homeobox
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Reverse transcription
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