Comparison of 454 Ultra-Deep Sequencing and Allele-Specific Real-Time PCR with Regard to the Detection of Emerging Drug-Resistant Minor HIV-1 Variants after Antiretroviral Prophylaxis for Vertical Transmission

Andrea Hauser; Claudia Kuecherer; Andrea Kunz; Piotr Wojtek Dabrowski; Aleksandar Radonić; Andreas Nitsche; Stefanie Theuring; Norbert Bannert; Julius Sewangi; Paulina Mbezi; Festo Dugange; Gundel Harms; Karolin Meixenberger

doi:10.1371/journal.pone.0140809

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Open Access

Peer-reviewed

Research Article

Comparison of 454 Ultra-Deep Sequencing and Allele-Specific Real-Time PCR with Regard to the Detection of Emerging Drug-Resistant Minor HIV-1 Variants after Antiretroviral Prophylaxis for Vertical Transmission

Andrea Hauser ,

* E-mail: hausera@rki.de

Affiliation HIV and other Retroviruses, Robert Koch-Institute, Berlin, Germany
⨯
Claudia Kuecherer,

Affiliation HIV and other Retroviruses, Robert Koch-Institute, Berlin, Germany
⨯
Andrea Kunz,

Affiliation Institute of Tropical Medicine and International Health, Charité - Universitätsmedizin Berlin, Berlin, Germany
⨯
Piotr Wojtek Dabrowski,

Affiliation Centre for Biological Threats and Special Pathogens 1, Highly Pathogenic Viruses, Robert Koch-Institute, Berlin, Germany
⨯
Aleksandar Radonić,

Affiliation Centre for Biological Threats and Special Pathogens 1, Highly Pathogenic Viruses, Robert Koch-Institute, Berlin, Germany
⨯
Andreas Nitsche,

Affiliation Centre for Biological Threats and Special Pathogens 1, Highly Pathogenic Viruses, Robert Koch-Institute, Berlin, Germany
⨯
Stefanie Theuring,

Affiliation Institute of Tropical Medicine and International Health, Charité - Universitätsmedizin Berlin, Berlin, Germany
⨯
Norbert Bannert,

Affiliation HIV and other Retroviruses, Robert Koch-Institute, Berlin, Germany
⨯
Julius Sewangi,

Affiliation Regional AIDS Control Program Mbeya Region, Ministry of Health and Social Welfare, Dar es Salaam, Tanzania
⨯
Paulina Mbezi,

Affiliation PMTCT Service Mbeya Region, Ministry of Health and Social Welfare, Dar es Salaam, Tanzania
⨯
Festo Dugange,

Affiliation Kyela District Hospital, Ministry of Health and Social Welfare, Dar es Salaam, Tanzania
⨯
Gundel Harms,

Affiliation Institute of Tropical Medicine and International Health, Charité - Universitätsmedizin Berlin, Berlin, Germany
⨯
Karolin Meixenberger

Affiliation HIV and other Retroviruses, Robert Koch-Institute, Berlin, Germany
⨯

Comparison of 454 Ultra-Deep Sequencing and Allele-Specific Real-Time PCR with Regard to the Detection of Emerging Drug-Resistant Minor HIV-1 Variants after Antiretroviral Prophylaxis for Vertical Transmission

Andrea Hauser,
Claudia Kuecherer,
Andrea Kunz,
Piotr Wojtek Dabrowski,
Aleksandar Radonić,
Andreas Nitsche,
Stefanie Theuring,
Norbert Bannert,
Julius Sewangi,
Paulina Mbezi

Published: October 15, 2015
https://doi.org/10.1371/journal.pone.0140809

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Allele-specific polymerase chain reaction
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Mutation detection
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HIV-1
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