Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards

Gertjan Kramer; Yvonne Woolerton; Jan P. van Straalen; Johannes P. C. Vissers; Nick Dekker; James I. Langridge; Robert J. Beynon; Dave Speijer; Auguste Sturk; Johannes M. F. G. Aerts

doi:10.1371/journal.pone.0140097

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Open Access

Peer-reviewed

Research Article

Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards

Gertjan Kramer ,

* E-mail: gertjan.kramer@embl.de

Current address: Genome Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯
Yvonne Woolerton,

Affiliation Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom
⨯
Jan P. van Straalen,

Affiliation Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯
Johannes P. C. Vissers,

Affiliation Waters Corporation, MS Technologies Centre, Manchester, United Kingdom
⨯
Nick Dekker,

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯
James I. Langridge,

Affiliation Waters Corporation, MS Technologies Centre, Manchester, United Kingdom
⨯
Robert J. Beynon,

Affiliation Centre for Proteome Research, Institute of Integrative Biology, University of Liverpool, Liverpool, United Kingdom
⨯
Dave Speijer,

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯
Auguste Sturk,

Affiliation Department of Clinical Chemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯
Johannes M. F. G. Aerts

Affiliation Department of Medical Biochemistry, Academic Medical Centre, University of Amsterdam, Amsterdam, the Netherlands
⨯

Accuracy and Reproducibility in Quantification of Plasma Protein Concentrations by Mass Spectrometry without the Use of Isotopic Standards

Gertjan Kramer,
Yvonne Woolerton,
Jan P. van Straalen,
Johannes P. C. Vissers,
Nick Dekker,
James I. Langridge,
Robert J. Beynon,
Dave Speijer,
Auguste Sturk,
Johannes M. F. G. Aerts

Published: October 16, 2015
https://doi.org/10.1371/journal.pone.0140097

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Blood plasma
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