Engineering Streptavidin and a Streptavidin-Binding Peptide with Infinite Binding Affinity and Reversible Binding Capability: Purification of a Tagged Recombinant Protein to High Purity via Affinity-Driven Thiol Coupling

Open Access

Peer-reviewed

Research Article

Dawson Fogen,

Affiliation Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
⨯
Sau-Ching Wu,

Affiliation Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
⨯
Kenneth Kai-Sing Ng,

Affiliation Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
⨯
Sui-Lam Wong

* E-mail: slwong@ucalgary.ca

Affiliation Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada
⨯

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Biotin
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Disulfide bonds
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Cysteine
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Protein complexes
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Gel electrophoresis
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Biosensors
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Polyacrylamide gel electrophoresis
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Thiols
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