Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach

Alexandra Pender; Isaac Garcia-Murillas; Sareena Rana; Rosalind J. Cutts; Gavin Kelly; Kerry Fenwick; Iwanka Kozarewa; David Gonzalez de Castro; Jaishree Bhosle; Mary O’Brien; Nicholas C. Turner; Sanjay Popat; Julian Downward

doi:10.1371/journal.pone.0139074

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Open Access

Peer-reviewed

Research Article

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach

Alexandra Pender,

Affiliations Lung Cancer Group, Division of Molecular Pathology, The Institute of Cancer Research, London, United Kingdom, Department of Medicine, The Royal Marsden NHS Foundation Trust, London, United Kingdom, The Francis Crick Institute, London, United Kingdom
⨯
Isaac Garcia-Murillas,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Sareena Rana,

Affiliation Lung Cancer Group, Division of Molecular Pathology, The Institute of Cancer Research, London, United Kingdom
⨯
Rosalind J. Cutts,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Gavin Kelly,

Affiliation The Francis Crick Institute, London, United Kingdom
⨯
Kerry Fenwick,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Iwanka Kozarewa,

Affiliation The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
David Gonzalez de Castro,

Affiliation The Centre for Molecular Pathology, The Institute of Cancer Research, Sutton, United Kingdom
⨯
Jaishree Bhosle,

Affiliation Department of Medicine, The Royal Marsden NHS Foundation Trust, London, United Kingdom
⨯
Mary O’Brien,

Affiliation Department of Medicine, The Royal Marsden NHS Foundation Trust, London, United Kingdom
⨯
Nicholas C. Turner,

Affiliations Department of Medicine, The Royal Marsden NHS Foundation Trust, London, United Kingdom, The Breakthrough Breast Cancer Research Centre, The Institute of Cancer Research, London, United Kingdom
⨯
Sanjay Popat,

Affiliation Department of Medicine, The Royal Marsden NHS Foundation Trust, London, United Kingdom
⨯
Julian Downward

* E-mail: julian.downward@crick.ac.uk

Affiliations Lung Cancer Group, Division of Molecular Pathology, The Institute of Cancer Research, London, United Kingdom, The Francis Crick Institute, London, United Kingdom
⨯

Efficient Genotyping of KRAS Mutant Non-Small Cell Lung Cancer Using a Multiplexed Droplet Digital PCR Approach

Alexandra Pender,
Isaac Garcia-Murillas,
Sareena Rana,
Rosalind J. Cutts,
Gavin Kelly,
Kerry Fenwick,
Iwanka Kozarewa,
David Gonzalez de Castro,
Jaishree Bhosle,
Mary O’Brien

Published: September 28, 2015
https://doi.org/10.1371/journal.pone.0139074

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Polymerase chain reaction
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Mutation detection
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Non-small cell lung cancer
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Next-generation sequencing
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Cross reactivity
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Dideoxy DNA sequencing
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DNA cloning
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Nucleotides
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