Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils

Gregory C. A. Amos; Chiara Borsetto; Paris Laskaris; Martin Krsek; Andrew E. Berry; Kevin K. Newsham; Leo Calvo-Bado; David A. Pearce; Carlos Vallin; Elizabeth M. H. Wellington

doi:10.1371/journal.pone.0138327

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Peer-reviewed

Research Article

Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils

Gregory C. A. Amos ,

Contributed equally to this work with: Gregory C. A. Amos, Chiara Borsetto

Current address: Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA, 92037, United States of America

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
Chiara Borsetto ,

Contributed equally to this work with: Gregory C. A. Amos, Chiara Borsetto

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
Paris Laskaris,

Current address: Insitut Pasteur, Infection and epidemiology department, Paris, 75015, France

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
Martin Krsek,

Current address: Department of Experimental Biology–Microbiology, Faculty of Science, Masaryk University, Brno, Czech Republic

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
Andrew E. Berry,

Current address: Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, CB10 1SA, United Kingdom

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
Kevin K. Newsham,

Affiliation Ecosystem Programme, British Antarctic Survey, Natural Environment Research Council, High Cross, Cambridge, CB3 OET, United Kingdom
⨯
Leo Calvo-Bado,

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯
David A. Pearce,

Current address: Applied Sciences, Faculty of Health and Life Sciences, Northumbria University, Newcastle-upon-Tyne, NE1 8ST, United Kingdom

Affiliation Ecosystem Programme, British Antarctic Survey, Natural Environment Research Council, High Cross, Cambridge, CB3 OET, United Kingdom
⨯
Carlos Vallin,

Affiliation Department of Biomedical Research, Center of Pharmaceutical Chemistry, Atabey, Playa, Havana, Cuba
⨯
Elizabeth M. H. Wellington

* E-mail: E.M.H.Wellington@warwick.ac.uk

Affiliation School of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
⨯

Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils

Gregory C. A. Amos,
Chiara Borsetto,
Paris Laskaris,
Martin Krsek,
Andrew E. Berry,
Kevin K. Newsham,
Leo Calvo-Bado,
David A. Pearce,
Carlos Vallin,
Elizabeth M. H. Wellington

Published: September 23, 2015
https://doi.org/10.1371/journal.pone.0138327

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