Intracellular Cytokine Staining and Flow Cytometry: Considerations for Application in Clinical Trials of Novel Tuberculosis Vaccines

• Steven G. Smith ,

  * E-mail: steven.smith@lshtm.ac.uk

  Affiliation Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom

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• Kaatje Smits,

  Affiliation Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles, Brussels, Belgium

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• Simone A. Joosten,

  Affiliation Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

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• Krista E. van Meijgaarden,

  Affiliation Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

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• Iman Satti,

  Affiliation The Jenner Institute, Old Road Campus Research Building, Roosevelt Drive, Oxford, United Kingdom

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• Helen A. Fletcher,

  Affiliations Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom, The Jenner Institute, Old Road Campus Research Building, Roosevelt Drive, Oxford, United Kingdom

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• Nadia Caccamo,

  Affiliation Biomedical Research Centre, Università di Palermo, Palermo, Italy, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy

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• Francesco Dieli,

  Affiliation Biomedical Research Centre, Università di Palermo, Palermo, Italy, Dipartimento di Biopatologia e Biotecnologie Mediche e Forensi, Università di Palermo, Palermo, Italy

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• Francoise Mascart,

  Affiliations Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles, Brussels, Belgium, Immunobiology Clinic, Hôpital Erasme, Université Libre de Bruxelles (U.L.B.), Brussels, Belgium

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• Helen McShane,

  Affiliation The Jenner Institute, Old Road Campus Research Building, Roosevelt Drive, Oxford, United Kingdom

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• Hazel M. Dockrell,

  Affiliation Department of Immunology and Infection, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, United Kingdom

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• Tom H. M. Ottenhoff,

  Affiliation Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands

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• TBVI TB Biomarker Working Group

  ¶Membership of the TBVI TB Biomarker Working Group is listed in the Acknowledgments.

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