Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies

• Maxim Kostylev ,

  Contributed equally to this work with: Maxim Kostylev, Anne E. Otwell

  * E-mail: ysuzuki@jcvi.org (YS), mkostyle@jcvi.org (MK)

  Affiliation Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, United States of America

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• Anne E. Otwell ,

  Contributed equally to this work with: Maxim Kostylev, Anne E. Otwell

  Affiliation Department of Microbiology, Cornell University, Ithaca, New York, United States of America

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• Ruth E. Richardson,

  Affiliation School of Civil and Environmental Engineering, Cornell University, Ithaca, New York, United States of America

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• Yo Suzuki

  * E-mail: ysuzuki@jcvi.org (YS), mkostyle@jcvi.org (MK)

  Affiliation Department of Synthetic Biology and Bioenergy, J. Craig Venter Institute, La Jolla, California, United States of America

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