An Integrated System for Precise Genome Modification in Escherichia coli

Open Access

Peer-reviewed

Research Article

Huseyin Tas,

Affiliations Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
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Cac T. Nguyen,

Affiliations Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯
Ravish Patel,

Affiliation Department of Molecular and Cellular Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
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Neil H. Kim,

Affiliations Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
⨯
Thomas E. Kuhlman

* E-mail: tkuhlman@illinois.edu

Affiliations Center for the Physics of Living Cells, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
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Polymerase chain reaction
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Homologous recombination
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Gene targeting
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Plasmid construction
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Genomics
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lac operon
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Oligonucleotides
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