Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

M. Crispo; A. P. Mulet; L. Tesson; N. Barrera; F. Cuadro; P. C. dos Santos-Neto; T. H. Nguyen; A. Crénéguy; L. Brusselle; I. Anegón; A. Menchaca

doi:10.1371/journal.pone.0136690

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Open Access

Peer-reviewed

Research Article

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

M. Crispo ,

* E-mail: crispo@pasteur.edu.uy (MC); ignacio.anegon@univ-nantes.fr (IA); menchaca.alejo@gmail.com (AM)

Affiliation Unidad de Animales Transgénicos y de Experimentación (UATE), Institut Pasteur de Montevideo, Montevideo, Uruguay
⨯
A. P. Mulet,

Affiliation Unidad de Animales Transgénicos y de Experimentación (UATE), Institut Pasteur de Montevideo, Montevideo, Uruguay
⨯
L. Tesson,

Affiliation INSERM UMR 1064, Center for Research in Transplantation and Immunology-ITUN, Nantes, France
⨯
N. Barrera,

Affiliation Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay
⨯
F. Cuadro,

Affiliation Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay
⨯
P. C. dos Santos-Neto,

Affiliation Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay
⨯
T. H. Nguyen,

Affiliation INSERM UMR 1064, Center for Research in Transplantation and Immunology-ITUN, Nantes, France
⨯
A. Crénéguy,

Affiliation INSERM UMR 1064, Center for Research in Transplantation and Immunology-ITUN, Nantes, France
⨯
L. Brusselle,

Affiliation INSERM UMR 1064, Center for Research in Transplantation and Immunology-ITUN, Nantes, France
⨯
I. Anegón ,

* E-mail: crispo@pasteur.edu.uy (MC); ignacio.anegon@univ-nantes.fr (IA); menchaca.alejo@gmail.com (AM)

Affiliation INSERM UMR 1064, Center for Research in Transplantation and Immunology-ITUN, Nantes, France
⨯
A. Menchaca

* E-mail: crispo@pasteur.edu.uy (MC); ignacio.anegon@univ-nantes.fr (IA); menchaca.alejo@gmail.com (AM)

Affiliation Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay
⨯

Efficient Generation of Myostatin Knock-Out Sheep Using CRISPR/Cas9 Technology and Microinjection into Zygotes

M. Crispo,
A. P. Mulet,
L. Tesson,
N. Barrera,
F. Cuadro,
P. C. dos Santos-Neto,
T. H. Nguyen,
A. Crénéguy,
L. Brusselle,
I. Anegón

Published: August 25, 2015
https://doi.org/10.1371/journal.pone.0136690

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