A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas

Haolin Liu; Janice White; Frances Crawford; Niyun Jin; Xiangwu Ju; Kangtai Liu; Chengyu Jiang; Philippa Marrack; Gongyi Zhang; John W. Kappler

doi:10.1371/journal.pone.0136613

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Open Access

Peer-reviewed

Research Article

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas

Haolin Liu,

Affiliations Howard Hughes Medical Institute, Denver, Colorado, United States of America, Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
Janice White,

Affiliations Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
Frances Crawford,

Affiliations Howard Hughes Medical Institute, Denver, Colorado, United States of America, Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
Niyun Jin,

Affiliations Howard Hughes Medical Institute, Denver, Colorado, United States of America, Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
Xiangwu Ju,

Affiliation State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China
⨯
Kangtai Liu,

Affiliation State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China
⨯
Chengyu Jiang,

Affiliation State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, Beijing, China
⨯
Philippa Marrack,

Affiliations Howard Hughes Medical Institute, Denver, Colorado, United States of America, Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
Gongyi Zhang,

Affiliations Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯
John W. Kappler

* E-mail: kapplerj@njhealth.org

Affiliations Howard Hughes Medical Institute, Denver, Colorado, United States of America, Department of Biomedical Research, National Jewish Health, Denver, Colorado, United States of America, Department of Immunology and Microbiology, University of Colorado Denver, Colorado, United States of America
⨯

A Rapid Method to Characterize Mouse IgG Antibodies and Isolate Native Antigen Binding IgG B Cell Hybridomas

Haolin Liu,
Janice White,
Frances Crawford,
Niyun Jin,
Xiangwu Ju,
Kangtai Liu,
Chengyu Jiang,
Philippa Marrack,
Gongyi Zhang,
John W. Kappler

Published: August 28, 2015
https://doi.org/10.1371/journal.pone.0136613

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Enzyme-linked immunoassays
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