Establishment and Validation of a Non-Radioactive Method for In Vitro Transcription Assay Using Primer Extension and Quantitative Real Time PCR

Open Access

Peer-reviewed

Research Article

Juan Wang,

Affiliation Institute of Biology and Medicine, Wuhan University of Science and Technology, Huangjiahu Campus, Wuhan, 430065, Hubei, China
⨯
Shasha Zhao,

Affiliation Institute of Biology and Medicine, Wuhan University of Science and Technology, Huangjiahu Campus, Wuhan, 430065, Hubei, China
⨯
Ying Zhou,

Affiliation Institute of Biology and Medicine, Wuhan University of Science and Technology, Huangjiahu Campus, Wuhan, 430065, Hubei, China
⨯
Yun Wei,

Affiliation Institute of Biology and Medicine, Wuhan University of Science and Technology, Huangjiahu Campus, Wuhan, 430065, Hubei, China
⨯
Wensheng Deng

* E-mail: dengwensheng@wust.edu.cn

Affiliation Institute of Biology and Medicine, Wuhan University of Science and Technology, Huangjiahu Campus, Wuhan, 430065, Hubei, China
⨯

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DNA transcription
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Primer extension
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Luciferase
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In vitro transcription assay
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Phenols
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Polymerase chain reaction
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Chemical precipitation
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Deoxyribonucleases
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