Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold

Anna V. Lomonosova; Elena V. Ovchinnikova; Alexei S. Kazakov; Alexander I. Denesyuk; Alexander D. Sofin; Roman V. Mikhailov; Andrei B. Ulitin; Tajib A. Mirzabekov; Eugene A. Permyakov; Sergei E. Permyakov

doi:10.1371/journal.pone.0134906

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Peer-reviewed

Research Article

Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold

Anna V. Lomonosova,

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Elena V. Ovchinnikova,

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Alexei S. Kazakov,

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Alexander I. Denesyuk,

Affiliations Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia, Department of Biosciences, Åbo Akademi University, Turku, 20520, Finland
⨯
Alexander D. Sofin,

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Roman V. Mikhailov,

Affiliation Antherix, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Andrei B. Ulitin,

Current Address: Biocad company, Lubuchany, Moscow region, 142380, Russia

Affiliation Antherix, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Tajib A. Mirzabekov,

Affiliations Antherix, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia, Biomirex Inc., 304 Pleasant Street, Watertown, Massachusetts, 02472, United States of America
⨯
Eugene A. Permyakov,

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯
Sergei E. Permyakov

* E-mail: permyakov.s@gmail.com

Affiliation Institute for Biological Instrumentation of the Russian Academy of Sciences, Institutskaya str., 7, Pushchino, Moscow region, 142290, Russia
⨯

Extremophilic 50S Ribosomal RNA-Binding Protein L35Ae as a Basis for Engineering of an Alternative Protein Scaffold

Anna V. Lomonosova,
Elena V. Ovchinnikova,
Alexei S. Kazakov,
Alexander I. Denesyuk,
Alexander D. Sofin,
Roman V. Mikhailov,
Andrei B. Ulitin,
Tajib A. Mirzabekov,
Eugene A. Permyakov,
Sergei E. Permyakov

Published: August 6, 2015
https://doi.org/10.1371/journal.pone.0134906

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Extremophiles
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Protein structure
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Polymerase chain reaction
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Mutagenesis
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Cell binding
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Thermal stability
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Fluorescence
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