An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products

Jin-ju Zhang; Jing-jing Tian; Shuang-shi Wei; Sheng-bao Duan; Hong-mei Wang; Ye-zhou Chen; Shao-hua Ding; Chun Zhang; Qing-lin Meng; Yong Li

doi:10.1371/journal.pone.0134743

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Open Access

Peer-reviewed

Research Article

An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products

Jin-ju Zhang,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Jing-jing Tian,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Shuang-shi Wei,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Sheng-bao Duan,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Hong-mei Wang,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Ye-zhou Chen,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Shao-hua Ding,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Chun Zhang,

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Qing-lin Meng ,

* E-mail: mengql@sibet.ac.cn

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯
Yong Li

Affiliation The Chinese Academy of Sciences Key Laboratory of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu, China
⨯

An Internal Reference Control Duplex Real-Time Polymerase Chain Reaction Assay for Detecting Bacterial Contamination in Blood Products

Jin-ju Zhang,
Jing-jing Tian,
Shuang-shi Wei,
Sheng-bao Duan,
Hong-mei Wang,
Ye-zhou Chen,
Shao-hua Ding,
Chun Zhang,
Qing-lin Meng,
Yong Li

Published: July 31, 2015
https://doi.org/10.1371/journal.pone.0134743

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Reverse transcriptase-polymerase chain reaction
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Polymerase chain reaction
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Platelets
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Blood plasma
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Ribosomal RNA
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Blood
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Sequence alignment
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