An IPTG Inducible Conditional Expression System for Mycobacteria

Sudha Ravishankar; Anisha Ambady; Haripriya Ramu; Naina Vinay Mudugal; Ragadeepthi Tunduguru; Anand Anbarasu; Umender K. Sharma; Vasan K. Sambandamurthy; Sudha Ramaiah

doi:10.1371/journal.pone.0134562

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Open Access

Peer-reviewed

Research Article

An IPTG Inducible Conditional Expression System for Mycobacteria

Sudha Ravishankar ,

* E-mail: mailsuravi01@gmail.com

Current address: School of Biosciences & Technology, VIT University, Vellore, Tamil Nadu, India

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Anisha Ambady,

Current address: Gangagen Biotechnologies Pvt. Ltd., Yeshwantpur, Bengaluru, Karnataka, India

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Haripriya Ramu,

Current address: Technology Transfer and Business Development, Oregon Health & Science University, Portland, Oregon, United States of America

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Naina Vinay Mudugal,

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Ragadeepthi Tunduguru,

Current address: Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, Indiana, United States of America

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Anand Anbarasu,

Affiliation School of Biosciences & Technology, VIT University, Vellore, Tamil Nadu, India
⨯
Umender K. Sharma,

Current address: Gangagen Biotechnologies Pvt. Ltd., Yeshwantpur, Bengaluru, Karnataka, India

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Vasan K. Sambandamurthy,

Current address: Mazumdar Shaw Center for Translational Research (MSCTR), Mazumdar Shaw Medical Foundation, Bengaluru, Karnataka, India

Affiliation AstraZeneca India Pvt Ltd, Bellary Road, Hebbal, Bengaluru, Karnataka, India
⨯
Sudha Ramaiah

Affiliation School of Biosciences & Technology, VIT University, Vellore, Tamil Nadu, India
⨯

An IPTG Inducible Conditional Expression System for Mycobacteria

Sudha Ravishankar,
Anisha Ambady,
Haripriya Ramu,
Naina Vinay Mudugal,
Ragadeepthi Tunduguru,
Anand Anbarasu,
Umender K. Sharma,
Vasan K. Sambandamurthy,
Sudha Ramaiah

Published: August 6, 2015
https://doi.org/10.1371/journal.pone.0134562

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Plasmid construction
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Gene expression
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Mycobacterium tuberculosis
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Gene targeting
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Gene cloning
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Mycobacteria
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Polymerase chain reaction
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Isoniazid
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