Spatial and Temporal Control of Cavitation Allows High In Vitro Transfection Efficiency in the Absence of Transfection Reagents or Contrast Agents

Kamel Chettab; Stéphanie Roux; Doriane Mathé; Emeline Cros-Perrial; Maxime Lafond; Cyril Lafon; Charles Dumontet; Jean-Louis Mestas

doi:10.1371/journal.pone.0134247

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Peer-reviewed

Research Article

Spatial and Temporal Control of Cavitation Allows High In Vitro Transfection Efficiency in the Absence of Transfection Reagents or Contrast Agents

Kamel Chettab ,

* E-mail: kchettab@yahoo.fr

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, CNRS UMR 5286, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, Caviskills SAS, Vaulx-en-Velin, France
⨯
Stéphanie Roux,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, CNRS UMR 5286, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France
⨯
Doriane Mathé,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, CNRS UMR 5286, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France
⨯
Emeline Cros-Perrial,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, CNRS UMR 5286, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France
⨯
Maxime Lafond,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, Inserm, U1032, LabTau, Lyon, F-69003, France
⨯
Cyril Lafon,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, Caviskills SAS, Vaulx-en-Velin, France, Inserm, U1032, LabTau, Lyon, F-69003, France
⨯
Charles Dumontet,

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, INSERM U1052, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France, CNRS UMR 5286, Centre de Recherche en Cancérologie de Lyon, 69008, Lyon, France
⨯
Jean-Louis Mestas

Affiliations Université de Lyon, 69000, Lyon, France, Université de Lyon 1, 69000, Lyon, France, Caviskills SAS, Vaulx-en-Velin, France, Inserm, U1032, LabTau, Lyon, F-69003, France
⨯

Spatial and Temporal Control of Cavitation Allows High In Vitro Transfection Efficiency in the Absence of Transfection Reagents or Contrast Agents

Kamel Chettab,
Stéphanie Roux,
Doriane Mathé,
Emeline Cros-Perrial,
Maxime Lafond,
Cyril Lafon,
Charles Dumontet,
Jean-Louis Mestas

Published: August 14, 2015
https://doi.org/10.1371/journal.pone.0134247

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Transfection
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HL60 cells
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BT474 cells
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Liposome-mediated transfection
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Cavitation
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Signal processing
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Flow cytometry
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Gene delivery
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