Optimisations and Challenges Involved in the Creation of Various Bioluminescent and Fluorescent Influenza A Virus Strains for In Vitro and In Vivo Applications

• Monique I. Spronken ,

  Contributed equally to this work with: Monique I. Spronken, Kirsty R. Short

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Kirsty R. Short ,

  Contributed equally to this work with: Monique I. Spronken, Kirsty R. Short

  Affiliations Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands, School of Biomedical Sciences, University of Queensland, Brisbane, Australia

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• Sander Herfst,

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Theo M. Bestebroer,

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Vincent P. Vaes,

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Barbara van der Hoeven,

  Affiliation Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Centre, Leiden, the Netherlands

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• Abraham J. Koster,

  Affiliation Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Centre, Leiden, the Netherlands

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• Gert-Jan Kremers,

  Affiliation Erasmus Optical Imaging Centre, Department of Pathology, Erasmus University Medical Centre, Rotterdam, the Netherlands

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• Dana P. Scott,

  Affiliation Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, United States of America

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• Alexander P. Gultyaev,

  Affiliations Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands, Leiden Institute of Advanced Computer Science, Leiden University, Leiden, the Netherlands

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• Erin M. Sorell,

  Affiliations Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands, Milken Institute School of Public Health, Department of Health Policy and Management, George Washington University, Washington, DC, United States of America

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• Miranda de Graaf,

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Montserrat Bárcena,

  Affiliation Department of Molecular Cell Biology, Section Electron Microscopy, Leiden University Medical Centre, Leiden, the Netherlands

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• Guus F. Rimmelzwaan,

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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• Ron A. Fouchier

  * E-mail: r.fouchier@erasmusmc.nl

  Affiliation Department of Viroscience, Erasmus Medical Centre, Rotterdam, the Netherlands

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