Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design

Jinfeng Zhang; Hao Shi; Linyu Xu; Xiaoyan Zhu; Xiangqian Li

doi:10.1371/journal.pone.0133824

Loading metrics

Open Access

Peer-reviewed

Research Article

Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design

Jinfeng Zhang,

Affiliations Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China, School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China
⨯
Hao Shi,

Affiliation Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China
⨯
Linyu Xu,

Affiliations Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China, School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China
⨯
Xiaoyan Zhu,

Affiliations Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China, School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China, Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, Jiangsu 223300, P. R. China
⨯
Xiangqian Li

* E-mail: lixq2002@126.com

Affiliations Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China, School of Life Science and Chemical Engineering, Huaiyin Institute of Technology, Huaian, Jiangsu 223003, P. R. China
⨯

Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design

Jinfeng Zhang,
Hao Shi,
Linyu Xu,
Xiaoyan Zhu,
Xiangqian Li

Published: July 28, 2015
https://doi.org/10.1371/journal.pone.0133824

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Protein structure
Is the Subject Area "Protein structure" applicable to this article?

Thanks for your feedback.
Enzyme structure
Is the Subject Area "Enzyme structure" applicable to this article?

Thanks for your feedback.
Cellulases
Is the Subject Area "Cellulases" applicable to this article?

Thanks for your feedback.
Cellulose
Is the Subject Area "Cellulose" applicable to this article?

Thanks for your feedback.
Crystal structure
Is the Subject Area "Crystal structure" applicable to this article?

Thanks for your feedback.
Enzymes
Is the Subject Area "Enzymes" applicable to this article?

Thanks for your feedback.
Site-directed mutagenesis
Is the Subject Area "Site-directed mutagenesis" applicable to this article?

Thanks for your feedback.
Hydrolysis
Is the Subject Area "Hydrolysis" applicable to this article?

Thanks for your feedback.