Removal of Contaminant DNA by Combined UV-EMA Treatment Allows Low Copy Number Detection of Clinically Relevant Bacteria Using Pan-Bacterial Real-Time PCR

• Bruce Humphrey ,

  * E-mail: bruce.humphrey@manchester.ac.uk

  Affiliations Institute of Inflammation and Repair, Faculty of Medical and Human Sciences, University of Manchester, Manchester, United Kingdom, Infection, Injury & inflammation Research Group, Salford Royal NHS Foundation Trust, Salford, United Kingdom

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• Neil McLeod,

  Affiliation Public Health England, Microbiology Services Division, Porton Down, Salisbury, United Kingdom

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• Carrie Turner,

  Affiliation Public Health England, Microbiology Services Division, Porton Down, Salisbury, United Kingdom

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• J. Mark Sutton,

  Affiliation Public Health England, Microbiology Services Division, Porton Down, Salisbury, United Kingdom

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• Paul M. Dark,

  Affiliations Institute of Inflammation and Repair, Faculty of Medical and Human Sciences, University of Manchester, Manchester, United Kingdom, Infection, Injury & inflammation Research Group, Salford Royal NHS Foundation Trust, Salford, United Kingdom

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• Geoffrey Warhurst

  Affiliations Institute of Inflammation and Repair, Faculty of Medical and Human Sciences, University of Manchester, Manchester, United Kingdom, Infection, Injury & inflammation Research Group, Salford Royal NHS Foundation Trust, Salford, United Kingdom

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