Nested PCR Biases in Interpreting Microbial Community Structure in 16S rRNA Gene Sequence Datasets

Open Access

Peer-reviewed

Research Article

Guoqin Yu ,

* E-mail: yug3@mail.nih.gov

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Doug Fadrosh,

Affiliation Institute of Genomic Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
James J. Goedert,

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
⨯
Jacques Ravel,

Affiliation Institute of Genomic Sciences, University of Maryland School of Medicine, Baltimore, Maryland, United States of America
⨯
Alisa M. Goldstein

Affiliation Division of Cancer Epidemiology and Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, United States of America
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Nested polymerase chain reaction
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Polymerase chain reaction
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Community structure
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Vagina
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Shannon index
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Ribosomal RNA
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Bacteria
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Microbiome
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