Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China

Lijuan Wu; Xiaomao Yin; Lei Zheng; Jianhua Zou; Ping Jin; Yanwei Hu; Timothy Kudinha; Fanrong Kong; Xu Chen; Qian Wang

doi:10.1371/journal.pone.0130664

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Peer-reviewed

Research Article

Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China

Lijuan Wu ,

Contributed equally to this work with: Lijuan Wu, Xiaomao Yin

Affiliations Laboratory Medical Centre, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China, Clinical Laboratory, Bao’an Maternity and Child Health Hospital, Shenzhen, Guangdong Province, China
⨯
Xiaomao Yin ,

Contributed equally to this work with: Lijuan Wu, Xiaomao Yin

Affiliation Clinical Laboratory, The Fifth Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong Province, China
⨯
Lei Zheng,

Affiliation Laboratory Medical Centre, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
⨯
Jianhua Zou,

Affiliation Clinical Laboratory, Bao’an Maternity and Child Health Hospital, Shenzhen, Guangdong Province, China
⨯
Ping Jin,

Affiliation Clinical Laboratory, Bao’an Maternity and Child Health Hospital, Shenzhen, Guangdong Province, China
⨯
Yanwei Hu,

Affiliation Laboratory Medical Centre, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
⨯
Timothy Kudinha,

Affiliation Charles Sturt University, Leeds Parade, Orange, New South Wales, Australia
⨯
Fanrong Kong,

Affiliation Centre for Infectious Diseases and Microbiology, Institute of Clinical Pathology and Medical Research–Pathology West, Westmead Hospital, New South Wales, Australia
⨯
Xu Chen,

Affiliation Clinical Laboratory, Bao’an Maternity and Child Health Hospital, Shenzhen, Guangdong Province, China
⨯
Qian Wang

* E-mail: nfyyWangQian@163.com

Affiliation Laboratory Medical Centre, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong Province, China
⨯

Practical Prediction of Ten Common Streptococcus pneumoniae Serotypes/Serogroups in One PCR Reaction by Multiplex Ligation-Dependent Probe Amplification and Melting Curve (MLPA-MC) Assay in Shenzhen, China

Lijuan Wu,
Xiaomao Yin,
Lei Zheng,
Jianhua Zou,
Ping Jin,
Yanwei Hu,
Timothy Kudinha,
Fanrong Kong,
Xu Chen,
Qian Wang

Published: July 7, 2015
https://doi.org/10.1371/journal.pone.0130664

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Polymerase chain reaction
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Melting
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Pneumococcus
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Probe hybridization
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Gel electrophoresis
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Fluorescent dyes
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Ligation assay
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DNA hybridization
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