Can DNA-Based Ecosystem Assessments Quantify Species Abundance? Testing Primer Bias and Biomass—Sequence Relationships with an Innovative Metabarcoding Protocol

Open Access

Peer-reviewed

Research Article

Vasco Elbrecht,

Affiliation Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany
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Florian Leese

* E-mail: florian.leese@rub.de

Affiliation Department of Animal Ecology, Evolution and Biodiversity, Ruhr University Bochum, Universitaetsstrasse 150, D-44801 Bochum, Germany
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Biomass
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DNA replication
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DNA extraction
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Polymerase chain reaction
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Taxonomy
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Biodiversity
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Ecosystems
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Nucleotide sequencing
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