A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis

Lauren Forbes; Katherine Ebsworth-Mojica; Louis DiDone; Shao-Gang Li; Joel S. Freundlich; Nancy Connell; Paul M. Dunman; Damian J. Krysan

doi:10.1371/journal.pone.0129234

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Peer-reviewed

Research Article

A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis

Lauren Forbes,

Affiliation Department of Microbiology/Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America
⨯
Katherine Ebsworth-Mojica,

Affiliation Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America
⨯
Louis DiDone,

Affiliation Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America
⨯
Shao-Gang Li,

Affiliation Department of Pharmacology, Rutgers University, Newark, New Jersey, 07103, United States of America
⨯
Joel S. Freundlich,

Affiliations Department of Pharmacology, Rutgers University, Newark, New Jersey, 07103, United States of America, Department of Physiology, Rutgers University, Newark, New Jersey, 07103, United States of America, Department of Medicine, Center for Emerging and Re-emerging Pathogens, Rutgers University, Newark, New Jersey, 07103, United States of America
⨯
Nancy Connell,

Affiliations Department of Physiology, Rutgers University, Newark, New Jersey, 07103, United States of America, Department of Medicine, Center for Emerging and Re-emerging Pathogens, Rutgers University, Newark, New Jersey, 07103, United States of America
⨯
Paul M. Dunman ,

* E-mail: damian_krysan@urmc.rochester.edu (DJK); paul_dunman@urmc.rochester.edu (PMD)

Affiliation Department of Microbiology/Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America
⨯
Damian J. Krysan

* E-mail: damian_krysan@urmc.rochester.edu (DJK); paul_dunman@urmc.rochester.edu (PMD)

Affiliations Department of Microbiology/Immunology, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America, Department of Pediatrics, University of Rochester School of Medicine and Dentistry, Rochester, New York, 14642, United States of America
⨯

A High Throughput Screening Assay for Anti-Mycobacterial Small Molecules Based on Adenylate Kinase Release as a Reporter of Cell Lysis

Lauren Forbes,
Katherine Ebsworth-Mojica,
Louis DiDone,
Shao-Gang Li,
Joel S. Freundlich,
Nancy Connell,
Paul M. Dunman,
Damian J. Krysan

Published: June 22, 2015
https://doi.org/10.1371/journal.pone.0129234

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