Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA

Brian T. Backstedt; Ozlem Buyuktanir; Janet Lindow; Elsio A. Wunder Jr.; Mitermayer G. Reis; Sahar Usmani-Brown; Michel Ledizet; Albert Ko; Utpal Pal

doi:10.1371/journal.pone.0128913

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Peer-reviewed

Research Article

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA

Brian T. Backstedt ,

Contributed equally to this work with: Brian T. Backstedt, Ozlem Buyuktanir

Affiliation Department of Veterinary Medicine and Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, United States of America
⨯
Ozlem Buyuktanir ,

Contributed equally to this work with: Brian T. Backstedt, Ozlem Buyuktanir

Current address: Department of Microbiology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey

Affiliation Department of Veterinary Medicine and Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, United States of America
⨯
Janet Lindow,

Affiliations Department of Epidemiology of Microbial Diseases, Yale University School of Public Health, New Haven, Connecticut, United States of America, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil
⨯
Elsio A. Wunder Jr.,

Affiliation Department of Epidemiology of Microbial Diseases, Yale University School of Public Health, New Haven, Connecticut, United States of America
⨯
Mitermayer G. Reis,

Affiliation Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil
⨯
Sahar Usmani-Brown,

Affiliation L2 Diagnostics, New Haven, Connecticut, United States of America
⨯
Michel Ledizet,

Affiliation L2 Diagnostics, New Haven, Connecticut, United States of America
⨯
Albert Ko,

Affiliations Department of Epidemiology of Microbial Diseases, Yale University School of Public Health, New Haven, Connecticut, United States of America, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil
⨯
Utpal Pal

* E-mail: upal@umd.edu

Affiliation Department of Veterinary Medicine and Virginia-Maryland Regional College of Veterinary Medicine, University of Maryland, College Park, Maryland, United States of America
⨯

Efficient Detection of Pathogenic Leptospires Using 16S Ribosomal RNA

Brian T. Backstedt,
Ozlem Buyuktanir,
Janet Lindow,
Elsio A. Wunder Jr.,
Mitermayer G. Reis,
Sahar Usmani-Brown,
Michel Ledizet,
Albert Ko,
Utpal Pal

Published: June 19, 2015
https://doi.org/10.1371/journal.pone.0128913

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Leptospira
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Bacterial pathogens
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Leptospirosis
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Blood
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Leptospira interrogans
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Polymerase chain reaction
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Diagnostic medicine
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Ribosomal RNA
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