In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4+ T Cells in Tissues

Open Access

Peer-reviewed

Research Article

In Situ Peptide-MHC-II Tetramer Staining of Antigen-Specific CD4⁺ T Cells in Tissues

Thamotharampillai Dileepan ,

Contributed equally to this work with: Thamotharampillai Dileepan, Hyeon O. Kim

Affiliation Department of Microbiology, University of Minnesota, Minneapolis, MN, United States of America
⨯
Hyeon O. Kim ,

Contributed equally to this work with: Thamotharampillai Dileepan, Hyeon O. Kim

Affiliation Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, United States of America
⨯
P. Patrick Cleary,

Affiliation Department of Microbiology, University of Minnesota, Minneapolis, MN, United States of America
⨯
Pamela J. Skinner

* E-mail: skinn002@umn.edu

Affiliation Department of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN, United States of America
⨯

T cells
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Cell staining
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Flow cytometry
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Signal amplification
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Spleen
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T helper cells
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B cells
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Group A streptococcal infection
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