Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme

Eva Csefalvay; Mikalai Lapkouski; Alena Guzanova; Ladislav Csefalvay; Tatsiana Baikova; Igor Shevelev; Vitali Bialevich; Katsiaryna Shamayeva; Pavel Janscak; Ivana Kuta Smatanova; Santosh Panjikar; Jannette Carey; Marie Weiserova; Rüdiger Ettrich

doi:10.1371/journal.pone.0128700

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Research Article

Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme

Eva Csefalvay,

Affiliation Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Mikalai Lapkouski,

Current address: Department of Cell and Molecular Biology, Karolinska Institute, 171 77 Stockholm, Sweden; Centre for Structural Systems Biology, DESY, 22607, Hamburg, Germany

Affiliation Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Alena Guzanova,

Affiliation Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic
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Ladislav Csefalvay,

Affiliation Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Tatsiana Baikova,

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Igor Shevelev †,

† Deceased.

Affiliation Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic
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Vitali Bialevich,

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Katsiaryna Shamayeva,

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Pavel Janscak,

Affiliations Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic, Institute of Molecular Cancer Research, University of Zürich, Wintherthurerstrasse 190, CH-8057 Zürich, Switzerland
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Ivana Kuta Smatanova,

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
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Santosh Panjikar,

Affiliations Australian Synchrotron, 800 Blackburn Road, Clayton VIC 3168, Australia, Department of Biochemistry and Molecular Biology, Monash University, Clayton Campus, Melbourne, VIC 3800 Australia
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Jannette Carey,

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Chemistry Department, Princeton University, Princeton, New Jersey 08544–1009, United States of America
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Marie Weiserova,

Affiliation Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Praha 4, Czech Republic
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Rüdiger Ettrich

* E-mail: ettrich@nh.cas.cz

Affiliations Center for Nanobiology and Structural Biology, Institute of Microbiology and Global Change Research Center, Academy of Sciences of the Czech Republic, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic, Faculty of Sciences, University of South Bohemia in Ceske Budejovice, Zamek 136, CZ-373 33 Nove Hrady, Czech Republic
⨯

Functional Coupling of Duplex Translocation to DNA Cleavage in a Type I Restriction Enzyme

Eva Csefalvay,
Mikalai Lapkouski,
Alena Guzanova,
Ladislav Csefalvay,
Tatsiana Baikova,
Igor Shevelev,
Vitali Bialevich,
Katsiaryna Shamayeva,
Pavel Janscak,
Ivana Kuta Smatanova

Published: June 3, 2015
https://doi.org/10.1371/journal.pone.0128700

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