Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches

Open Access

Peer-reviewed

Research Article

Stefan J. Green ,

* E-mail: GreenDNA@uic.edu

Affiliations DNA Services Facility, Research Resources Center, University of Illinois at Chicago, Chicago, Illinois, United States of America, Dept. of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois, United States of America
⨯
Raghavee Venkatramanan,

Affiliations DNA Services Facility, Research Resources Center, University of Illinois at Chicago, Chicago, Illinois, United States of America, Dept. of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States of America
⨯
Ankur Naqib

Affiliations DNA Services Facility, Research Resources Center, University of Illinois at Chicago, Chicago, Illinois, United States of America, Dept. of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States of America
⨯

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Polymerase chain reaction
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DNA annealing
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Genomics
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Signal amplification
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Community structure
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Genetic annealing
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Ribosomal RNA
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