Rapid and Specific Enrichment of Culturable Gram Negative Bacteria Using Non-Lethal Copper-Free Click Chemistry Coupled with Magnetic Beads Separation

Emilie Fugier; Audrey Dumont; Annie Malleron; Enora Poquet; Jordi Mas Pons; Aurélie Baron; Boris Vauzeilles; Sam Dukan

doi:10.1371/journal.pone.0127700

Loading metrics

Open Access

Peer-reviewed

Research Article

Rapid and Specific Enrichment of Culturable Gram Negative Bacteria Using Non-Lethal Copper-Free Click Chemistry Coupled with Magnetic Beads Separation

Emilie Fugier,

Current address: Click4Tag, Zone Luminy Biotech, Case 922, 163 avenue de Luminy, 13009, Marseille, France

Affiliation Aix Marseille Université, Laboratoire de Chimie Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier—13402, Marseille, France
⨯
Audrey Dumont,

Current address: Click4Tag, Zone Luminy Biotech, Case 922, 163 avenue de Luminy, 13009, Marseille, France

Affiliation Aix Marseille Université, Laboratoire de Chimie Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier—13402, Marseille, France
⨯
Annie Malleron,

Affiliation CNRS and Univ Paris-Sud, Laboratoire de Synthèse de Biomolécules, Institut de Chimie Moléculaire et des Matériaux d’Orsay, UMR 8182, 91405, Orsay, France
⨯
Enora Poquet,

Affiliation Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles du CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France
⨯
Jordi Mas Pons,

Affiliation Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles du CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France
⨯
Aurélie Baron,

Affiliation Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles du CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France
⨯
Boris Vauzeilles ,

* E-mail: sam.dukan@click4tag.com (SD); boris.vauzeilles@cnrs.fr (BV)

Affiliations CNRS and Univ Paris-Sud, Laboratoire de Synthèse de Biomolécules, Institut de Chimie Moléculaire et des Matériaux d’Orsay, UMR 8182, 91405, Orsay, France, Centre de Recherche de Gif, Institut de Chimie des Substances Naturelles du CNRS, Avenue de la Terrasse, 91198, Gif-sur-Yvette, France
⨯
Sam Dukan

* E-mail: sam.dukan@click4tag.com (SD); boris.vauzeilles@cnrs.fr (BV)

Current address: Click4Tag, Zone Luminy Biotech, Case 922, 163 avenue de Luminy, 13009, Marseille, France

Affiliation Aix Marseille Université, Laboratoire de Chimie Bactérienne (UMR 7283), Institut de Microbiologie de la Méditerranée (IMM), CNRS, 31 Chemin Joseph Aiguier—13402, Marseille, France
⨯

Rapid and Specific Enrichment of Culturable Gram Negative Bacteria Using Non-Lethal Copper-Free Click Chemistry Coupled with Magnetic Beads Separation

Emilie Fugier,
Audrey Dumont,
Annie Malleron,
Enora Poquet,
Jordi Mas Pons,
Aurélie Baron,
Boris Vauzeilles,
Sam Dukan

Published: June 10, 2015
https://doi.org/10.1371/journal.pone.0127700

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Bacillus subtilis
Is the Subject Area "Bacillus subtilis" applicable to this article?

Thanks for your feedback.
Bacterial biochemistry
Is the Subject Area "Bacterial biochemistry" applicable to this article?

Thanks for your feedback.
Gram negative bacteria
Is the Subject Area "Gram negative bacteria" applicable to this article?

Thanks for your feedback.
Chemistry
Is the Subject Area "Chemistry" applicable to this article?

Thanks for your feedback.
Alkynes
Is the Subject Area "Alkynes" applicable to this article?

Thanks for your feedback.
Bacteria
Is the Subject Area "Bacteria" applicable to this article?

Thanks for your feedback.
Enzyme-linked immunoassays
Is the Subject Area "Enzyme-linked immunoassays" applicable to this article?

Thanks for your feedback.
Metabolic labeling
Is the Subject Area "Metabolic labeling" applicable to this article?

Thanks for your feedback.