Structure Elucidation of the Metabolites of 2', 3', 5'-Tri-O-Acetyl-N6-(3-Hydroxyphenyl) Adenosine in Rat Urine by HPLC-DAD, ESI-MS and Off-Line Microprobe NMR

Wei Guo; Mengxia Jin; Zhaoxia Miao; Kai Qu; Xia Liu; Peicheng Zhang; Hailin Qin; Haibo Zhu; Yinghong Wang

doi:10.1371/journal.pone.0127583

Loading metrics

Open Access

Peer-reviewed

Research Article

Structure Elucidation of the Metabolites of 2', 3', 5'-Tri-O-Acetyl-N₆-(3-Hydroxyphenyl) Adenosine in Rat Urine by HPLC-DAD, ESI-MS and Off-Line Microprobe NMR

Wei Guo ,

Contributed equally to this work with: Wei Guo, Mengxia Jin

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Mengxia Jin ,

Contributed equally to this work with: Wei Guo, Mengxia Jin

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Zhaoxia Miao,

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Kai Qu,

Affiliations State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Xia Liu,

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Peicheng Zhang,

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Hailin Qin,

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Haibo Zhu ,

* E-mail: wyh@imm.ac.cn (YW); zhuhaibo@imm.ac.cn (HZ)

Affiliations State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯
Yinghong Wang

* E-mail: wyh@imm.ac.cn (YW); zhuhaibo@imm.ac.cn (HZ)

Affiliation State Key Laboratory of Bioactive Substance and Function of Natural Medicines & Ministry of Health Key Laboratory of Biosynthesis of Natural Products, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
⨯

Structure Elucidation of the Metabolites of 2', 3', 5'-Tri-O-Acetyl-N₆-(3-Hydroxyphenyl) Adenosine in Rat Urine by HPLC-DAD, ESI-MS and Off-Line Microprobe NMR

Wei Guo,
Mengxia Jin,
Zhaoxia Miao,
Kai Qu,
Xia Liu,
Peicheng Zhang,
Hailin Qin,
Haibo Zhu,
Yinghong Wang

Published: June 1, 2015
https://doi.org/10.1371/journal.pone.0127583

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

NMR spectroscopy
Is the Subject Area "NMR spectroscopy" applicable to this article?

Thanks for your feedback.
Metabolites
Is the Subject Area "Metabolites" applicable to this article?

Thanks for your feedback.
Adenosine
Is the Subject Area "Adenosine" applicable to this article?

Thanks for your feedback.
Urine
Is the Subject Area "Urine" applicable to this article?

Thanks for your feedback.
Adenine
Is the Subject Area "Adenine" applicable to this article?

Thanks for your feedback.
High performance liquid chromatography
Is the Subject Area "High performance liquid chromatography" applicable to this article?

Thanks for your feedback.
Nuclear magnetic resonance
Is the Subject Area "Nuclear magnetic resonance" applicable to this article?

Thanks for your feedback.
Metabolic pathways
Is the Subject Area "Metabolic pathways" applicable to this article?

Thanks for your feedback.