In-Frame Amber Stop Codon Replacement Mutagenesis for the Directed Evolution of Proteins Containing Non-Canonical Amino Acids: Identification of Residues Open to Bio-Orthogonal Modification

• James A. J. Arpino ,

  Current address: Centre for Synthetic Biology and Innovation, Imperial College London, London, United Kingdom

  ¶‡ These authors are joint first authors on this work.

  Affiliation School of Biosciences, Main Building, Park Place, Cardiff University, Cardiff, CF10 3AT, United Kingdom

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• Amy J. Baldwin ,

  ¶‡ These authors are joint first authors on this work.

  Affiliation School of Biosciences, Main Building, Park Place, Cardiff University, Cardiff, CF10 3AT, United Kingdom

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• Adam R. McGarrity,

  Affiliation School of Biosciences, Main Building, Park Place, Cardiff University, Cardiff, CF10 3AT, United Kingdom

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• Eric M. Tippmann,

  Affiliation Department of Chemistry, Indiana–Purdue University Fort Wayne, Fort Wayne, Indiana, 46815, United States of America

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• D. Dafydd Jones

  * E-mail: jonesdd@cf.ac.uk

  Affiliation School of Biosciences, Main Building, Park Place, Cardiff University, Cardiff, CF10 3AT, United Kingdom

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