Monitoring β-arrestin recruitment via β-lactamase enzyme fragment complementation: purification of peptide E as a low-affinity ligand for mammalian bombesin receptors

• Yuichi Ikeda ,

  Contributed equally to this work with: Yuichi Ikeda, Hidetoshi Kumagai

  * E-mail: yikeda-tky@umin.ac.jp

  Affiliations Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan, Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America

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• Hidetoshi Kumagai ,

  Contributed equally to this work with: Yuichi Ikeda, Hidetoshi Kumagai

  Affiliations Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan, Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, Department of Advanced Clinical Science and Therapeutics, University of Tokyo, Tokyo, Japan

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• Hiroaki Okazaki,

  Affiliation Department of Diabetes and Metabolic Diseases, University of Tokyo, Tokyo, Japan

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• Mitsuhiro Fujishiro,

  Affiliation Department of Gastroenterology, University of Tokyo, Tokyo, Japan

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• Yoshihiro Motozawa,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Seitaro Nomura,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Norifumi Takeda,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Haruhiro Toko,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Eiki Takimoto,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Hiroshi Akazawa,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Hiroyuki Morita,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Jun-ichi Suzuki,

  Affiliation Department of Advanced Clinical Science and Therapeutics, University of Tokyo, Tokyo, Japan

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• Tsutomu Yamazaki,

  Affiliation Clinical Research Support Center, University of Tokyo, Tokyo, Japan

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• Issei Komuro,

  Affiliation Department of Cardiovascular Medicine, University of Tokyo, Tokyo, Japan

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• Masashi Yanagisawa

  Affiliations Department of Molecular Genetics, Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America, International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Japan

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