Bias in Ligation-Based Small RNA Sequencing Library Construction Is Determined by Adaptor and RNA Structure

Open Access

Peer-reviewed

Research Article

Ryan T. Fuchs,

Affiliation RNA Research Division, New England Biolabs Incorporated, Ipswich, Massachusetts, United States of America
⨯
Zhiyi Sun,

Affiliation RNA Research Division, New England Biolabs Incorporated, Ipswich, Massachusetts, United States of America
⨯
Fanglei Zhuang,

Current address: BioRad Laboratories Incorporated Digital Biology Center, Cambridge, Massachusetts, United States of America

Affiliation RNA Research Division, New England Biolabs Incorporated, Ipswich, Massachusetts, United States of America
⨯
G. Brett Robb

* E-mail: robb@neb.com

Affiliation RNA Research Division, New England Biolabs Incorporated, Ipswich, Massachusetts, United States of America
⨯

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MicroRNAs
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RNA sequencing
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RNA structure
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cDNA libraries
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Oligonucleotides
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Ligation assay
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Polymerase chain reaction
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Ligases
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