Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1

Masahito Asada; Kazuhide Yahata; Hassan Hakimi; Naoaki Yokoyama; Ikuo Igarashi; Osamu Kaneko; Carlos E. Suarez; Shin-ichiro Kawazu

doi:10.1371/journal.pone.0125993

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Peer-reviewed

Research Article

Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1

Masahito Asada,

Affiliations Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Sakamoto, Nagasaki, Japan, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
⨯
Kazuhide Yahata,

Affiliation Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Sakamoto, Nagasaki, Japan
⨯
Hassan Hakimi,

Affiliations Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Sakamoto, Nagasaki, Japan, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
⨯
Naoaki Yokoyama,

Affiliation National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
⨯
Ikuo Igarashi,

Affiliation National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
⨯
Osamu Kaneko,

Affiliation Department of Protozoology, Institute of Tropical Medicine (NEKKEN), Nagasaki University, Sakamoto, Nagasaki, Japan
⨯
Carlos E. Suarez ,

Contributed equally to this work with: Carlos E. Suarez, Shin-ichiro Kawazu

* E-mail: E-mail: ces@vetmed.wsu.edu (CES); skawazu@obihiro.ac.jp (S-IK)

Affiliations Program in Vector-Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, United States of America, Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, Washington, United States of America
⨯
Shin-ichiro Kawazu

Contributed equally to this work with: Carlos E. Suarez, Shin-ichiro Kawazu

* E-mail: E-mail: ces@vetmed.wsu.edu (CES); skawazu@obihiro.ac.jp (S-IK)

Affiliation National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Japan
⨯

Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1

Masahito Asada,
Kazuhide Yahata,
Hassan Hakimi,
Naoaki Yokoyama,
Ikuo Igarashi,
Osamu Kaneko,
Carlos E. Suarez,
Shin-ichiro Kawazu

Published: May 11, 2015
https://doi.org/10.1371/journal.pone.0125993

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