Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs

Open Access

Peer-reviewed

Research Article

Thimma R. Reddy,

Affiliation Department of Biochemistry, University of Leicester, Leicester, LE1 9HN, United Kingdom
⨯
Emma J. Kelsall,

Affiliation Department of Biochemistry, University of Leicester, Leicester, LE1 9HN, United Kingdom
⨯
Léna M. S. Fevat,

Affiliation Center for Fisheries, Environment and Aquaculture Sciences, Lowestoft, NR33 0HT, United Kingdom
⨯
Sarah E. Munson,

Affiliation ES Cell Facility, Center for Core Biotechnology Services, Leicester, LE1 9HN, United Kingdom
⨯
Shaun M. Cowley

* E-mail: smc57@le.ac.uk

Affiliation Department of Biochemistry, University of Leicester, Leicester, LE1 9HN, United Kingdom
⨯

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Homologous recombination
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DNA recombination
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DNA repair
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Recombinant proteins
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Subcloning
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Polymerase chain reaction
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Recombination-based assay
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Antibiotics
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