Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal

Ganeshan Sivanandhan; Gnajothi Kapil Dev; Jeevaraj Theboral; Natesan Selvaraj; Andy Ganapathi; Markandan Manickavasagam

doi:10.1371/journal.pone.0124693

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Peer-reviewed

Research Article

Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal

Ganeshan Sivanandhan ,

* E-mail: manickbiotech@gmail.com (MM); gsivabdu@gmail.com (GS)

Affiliations Plant Molecular Biology Laboratory, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India, Molecular Genetics and Genomics Laboratory, Department of Horticulture, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305 764, South Korea
⨯
Gnajothi Kapil Dev,

Affiliation Plant Molecular Biology Laboratory, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
⨯
Jeevaraj Theboral,

Affiliation Plant Molecular Biology Laboratory, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
⨯
Natesan Selvaraj,

Affiliation Plant Tissue Culture Laboratory, Department of Botany, Periyar E.V.R College (Autonomous), Tiruchirappalli 620 023, Tamil Nadu, India
⨯
Andy Ganapathi,

Affiliation Plant Molecular Biology Laboratory, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
⨯
Markandan Manickavasagam

* E-mail: manickbiotech@gmail.com (MM); gsivabdu@gmail.com (GS)

Affiliation Plant Molecular Biology Laboratory, Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India
⨯

Sonication, Vacuum Infiltration and Thiol Compounds Enhance the Agrobacterium-Mediated Transformation Frequency of Withania somnifera (L.) Dunal

Ganeshan Sivanandhan,
Gnajothi Kapil Dev,
Jeevaraj Theboral,
Natesan Selvaraj,
Andy Ganapathi,
Markandan Manickavasagam

Published: April 30, 2015
https://doi.org/10.1371/journal.pone.0124693

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