Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature

Open Access

Peer-reviewed

Research Article

Chi-Yen Lin,

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Zhen Huang,

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Wei Wen,

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Andrew Wu,

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Congzhou Wang,

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Li Niu

* E-mail: lniu@albany.edu

Affiliation Department of Chemistry, and Center for Neuroscience Research, University at Albany, SUNY, Albany, New York, 12222, United States of America
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Transfection
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Fluorescence imaging
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Protein expression
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Recombinant proteins
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Bright field imaging
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Calcium phosphate transfection
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Green fluorescent protein
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Membrane proteins
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