Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

Weiming Hao; Lujuan Fan; Qianqian Chen; Xiaoxiang Chen; Sichao Zhang; Ke Lan; Jian Lu; Chiyu Zhang

doi:10.1371/journal.pone.0123468

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Open Access

Peer-reviewed

Research Article

Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

Weiming Hao ,

Contributed equally to this work with: Weiming Hao, Lujuan Fan

Affiliations Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China, Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
⨯
Lujuan Fan ,

Contributed equally to this work with: Weiming Hao, Lujuan Fan

Affiliations Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China, Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
⨯
Qianqian Chen,

Affiliation Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
⨯
Xiaoxiang Chen,

Affiliation Department of Gynecologic Oncology, Jiangsu Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu, China
⨯
Sichao Zhang,

Affiliation Huzhou Center for Disease Control and Prevention, Huzhou, Zhejiang, China
⨯
Ke Lan,

Affiliation Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
⨯
Jian Lu ,

* E-mail: zhangcy1999@ips.ac.cn (CZ); lujian@ujs.edu.cn (JL)

Affiliations Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, China, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China
⨯
Chiyu Zhang

* E-mail: zhangcy1999@ips.ac.cn (CZ); lujian@ujs.edu.cn (JL)

Affiliation Pathogen Diagnostic Center, Institut Pasteur of Shanghai, Chinese Academy of Sciences, Shanghai, China
⨯

Modified Proofreading PCR for Detection of Point Mutations, Insertions and Deletions Using a ddNTP-Blocked Primer

Weiming Hao,
Lujuan Fan,
Qianqian Chen,
Xiaoxiang Chen,
Sichao Zhang,
Ke Lan,
Jian Lu,
Chiyu Zhang

Published: April 27, 2015
https://doi.org/10.1371/journal.pone.0123468

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