Fourth-Generation Epac-Based FRET Sensors for cAMP Feature Exceptional Brightness, Photostability and Dynamic Range: Characterization of Dedicated Sensors for FLIM, for Ratiometry and with High Affinity

• Jeffrey Klarenbeek,

  Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, van Leeuwenhoek Centre of Advanced Microscopy, Amsterdam, The Netherlands

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• Joachim Goedhart,

  Affiliations Section of Molecular Cytology, Swammerdam Institute for Life Sciences, University of Amsterdam, The Netherlands, van Leeuwenhoek Centre of Advanced Microscopy, Amsterdam, The Netherlands

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• Aernoud van Batenburg,

  Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, van Leeuwenhoek Centre of Advanced Microscopy, Amsterdam, The Netherlands

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• Daniella Groenewald,

  Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, van Leeuwenhoek Centre of Advanced Microscopy, Amsterdam, The Netherlands

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• Kees Jalink

  * E-mail: k.jalink@nki.nl

  Affiliations Division of Cell Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands, van Leeuwenhoek Centre of Advanced Microscopy, Amsterdam, The Netherlands

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