A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species

Hirohito Ogawa; Daisuke Fujikura; Miyuki Ohnuma; Naomi Ohnishi; Bernard M. Hang'ombe; Hitomi Mimuro; Takayuki Ezaki; Aaron S. Mweene; Hideaki Higashi

doi:10.1371/journal.pone.0122004

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Peer-reviewed

Research Article

A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species

Hirohito Ogawa,

Affiliations Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia, Department of Disease Control, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia
⨯
Daisuke Fujikura,

Affiliation Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
⨯
Miyuki Ohnuma,

Affiliation Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
⨯
Naomi Ohnishi,

Affiliation Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan
⨯
Bernard M. Hang'ombe,

Affiliation Department of Paraclinical Studies, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia
⨯
Hitomi Mimuro,

Affiliations Division of Bacteriology, Department of Infectious Diseases Control, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, Tokyo, Japan, Pathogenic Microbes Repository Unit, International Research Center for Infectious Diseases, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
⨯
Takayuki Ezaki,

Affiliation Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Gifu, Japan
⨯
Aaron S. Mweene,

Affiliation Department of Disease Control, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia
⨯
Hideaki Higashi

* E-mail: hidea-hi@czc.hokudai.ac.jp

Affiliations Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia, Department of Disease Control, School of Veterinary Medicine, The University of Zambia, Lusaka, Zambia, Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan, Global Station for Zoonosis Control, Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan
⨯

A Novel Multiplex PCR Discriminates Bacillus anthracis and Its Genetically Related Strains from Other Bacillus cereus Group Species

Hirohito Ogawa,
Daisuke Fujikura,
Miyuki Ohnuma,
Naomi Ohnishi,
Bernard M. Hang'ombe,
Hitomi Mimuro,
Takayuki Ezaki,
Aaron S. Mweene,
Hideaki Higashi

Published: March 16, 2015
https://doi.org/10.1371/journal.pone.0122004

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Bacillus anthracis
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Polymerase chain reaction
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Ribosomal RNA
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