Systematic Mutagenesis of Genes Encoding Predicted Autotransported Proteins of Burkholderia pseudomallei Identifies Factors Mediating Virulence in Mice, Net Intracellular Replication and a Novel Protein Conferring Serum Resistance

Natalie R. Lazar Adler; Mark P. Stevens; Rachel E. Dean; Richard J. Saint; Depesh Pankhania; Joann L. Prior; Timothy P. Atkins; Bianca Kessler; Arnone Nithichanon; Ganjana Lertmemongkolchai; Edouard E. Galyov

doi:10.1371/journal.pone.0121271

Loading metrics

Open Access

Peer-reviewed

Research Article

Systematic Mutagenesis of Genes Encoding Predicted Autotransported Proteins of Burkholderia pseudomallei Identifies Factors Mediating Virulence in Mice, Net Intracellular Replication and a Novel Protein Conferring Serum Resistance

Natalie R. Lazar Adler,

Affiliation Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom
⨯
Mark P. Stevens,

Affiliation The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, United Kingdom
⨯
Rachel E. Dean,

Affiliation Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, United Kingdom
⨯
Richard J. Saint,

Affiliation Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, United Kingdom
⨯
Depesh Pankhania,

Affiliation Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom
⨯
Joann L. Prior,

Affiliation Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, United Kingdom
⨯
Timothy P. Atkins,

Affiliations Biomedical Sciences, Defence Science and Technology Laboratory, Porton Down, United Kingdom, School of Biosciences, Geoffrey Pope Building, University of Exeter, Exeter, United Kingdom
⨯
Bianca Kessler,

Affiliation The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
⨯
Arnone Nithichanon,

Affiliation The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
⨯
Ganjana Lertmemongkolchai,

Affiliation The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
⨯
Edouard E. Galyov

* E-mail: eg98@leicester.ac.uk

Affiliation Department of Infection, Immunity and Inflammation, University of Leicester, Leicester, United Kingdom
⨯

Systematic Mutagenesis of Genes Encoding Predicted Autotransported Proteins of Burkholderia pseudomallei Identifies Factors Mediating Virulence in Mice, Net Intracellular Replication and a Novel Protein Conferring Serum Resistance

Natalie R. Lazar Adler,
Mark P. Stevens,
Rachel E. Dean,
Richard J. Saint,
Depesh Pankhania,
Joann L. Prior,
Timothy P. Atkins,
Bianca Kessler,
Arnone Nithichanon,
Ganjana Lertmemongkolchai

Published: April 1, 2015
https://doi.org/10.1371/journal.pone.0121271

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Complement system
Is the Subject Area "Complement system" applicable to this article?

Thanks for your feedback.
Melioidosis
Is the Subject Area "Melioidosis" applicable to this article?

Thanks for your feedback.
Intracellular pathogens
Is the Subject Area "Intracellular pathogens" applicable to this article?

Thanks for your feedback.
Phenotypes
Is the Subject Area "Phenotypes" applicable to this article?

Thanks for your feedback.
Recombinant proteins
Is the Subject Area "Recombinant proteins" applicable to this article?

Thanks for your feedback.
Lectins
Is the Subject Area "Lectins" applicable to this article?

Thanks for your feedback.
Polymerase chain reaction
Is the Subject Area "Polymerase chain reaction" applicable to this article?

Thanks for your feedback.
Protein expression
Is the Subject Area "Protein expression" applicable to this article?

Thanks for your feedback.