tRNAGlu Increases the Affinity of Glutamyl-tRNA Synthetase for Its Inhibitor Glutamyl-Sulfamoyl-Adenosine, an Analogue of the Aminoacylation Reaction Intermediate Glutamyl-AMP: Mechanistic and Evolutionary Implications

Sébastien P. Blais; Jack A. Kornblatt; Xavier Barbeau; Guillaume Bonnaure; Patrick Lagüe; Robert Chênevert; Jacques Lapointe

doi:10.1371/journal.pone.0121043

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Research Article

tRNA^Glu Increases the Affinity of Glutamyl-tRNA Synthetase for Its Inhibitor Glutamyl-Sulfamoyl-Adenosine, an Analogue of the Aminoacylation Reaction Intermediate Glutamyl-AMP: Mechanistic and Evolutionary Implications

Sébastien P. Blais ,

* E-mail: sebastien.blais@bcm.ulaval.ca (SPB); jacques.lapointe@bcm.ulaval.ca (JL)

Affiliations Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, Canada, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯
Jack A. Kornblatt,

Affiliation Department of Biology, Centre for Structural and Functional Genomics, Faculty of Arts and Science, Concordia University, Montréal, Canada
⨯
Xavier Barbeau,

Affiliations Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, Canada, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Canada, Département de Chimie, Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯
Guillaume Bonnaure,

Affiliations Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, Canada, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯
Patrick Lagüe,

Affiliations Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, Canada, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯
Robert Chênevert,

Affiliations Département de Chimie, Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯
Jacques Lapointe

* E-mail: sebastien.blais@bcm.ulaval.ca (SPB); jacques.lapointe@bcm.ulaval.ca (JL)

Affiliations Département de Biochimie, de Microbiologie et de Bio-informatique, Université Laval, Québec, Canada, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, Canada, The Quebec Network for Research on Protein Function, Structure, and Engineering (PROTEO), Québec, Canada
⨯

tRNA^Glu Increases the Affinity of Glutamyl-tRNA Synthetase for Its Inhibitor Glutamyl-Sulfamoyl-Adenosine, an Analogue of the Aminoacylation Reaction Intermediate Glutamyl-AMP: Mechanistic and Evolutionary Implications

Sébastien P. Blais,
Jack A. Kornblatt,
Xavier Barbeau,
Guillaume Bonnaure,
Patrick Lagüe,
Robert Chênevert,
Jacques Lapointe

Published: April 10, 2015
https://doi.org/10.1371/journal.pone.0121043

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