High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Fei Wang; Xiaojuan Wang; Xiaolan Yu; Lin Fu; Yunyun Liu; Lixin Ma; Chao Zhai

doi:10.1371/journal.pone.0120458

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Peer-reviewed

Research Article

High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Fei Wang,

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Xiaojuan Wang,

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Xiaolan Yu,

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Lin Fu,

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Yunyun Liu,

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Lixin Ma ,

* E-mail: malixing@hubu.edu.cn (LM); chaozhai@hotmail.com (CZ)

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯
Chao Zhai

* E-mail: malixing@hubu.edu.cn (LM); chaozhai@hotmail.com (CZ)

Affiliation Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, People’s Republic of China
⨯

High-Level Expression of Endo-β-N-Acetylglucosaminidase H from Streptomyces plicatus in Pichia pastoris and Its Application for the Deglycosylation of Glycoproteins

Fei Wang,
Xiaojuan Wang,
Xiaolan Yu,
Lin Fu,
Yunyun Liu,
Lixin Ma,
Chao Zhai

Published: March 17, 2015
https://doi.org/10.1371/journal.pone.0120458

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Recombinant proteins
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Glycoproteins
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Ribonucleases
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Deoxyribonucleases
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Polymerase chain reaction
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Pichia pastoris
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RNA denaturation
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Glycosylation
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