A Method for Combining RNAscope In Situ Hybridization with Immunohistochemistry in Thick Free-Floating Brain Sections and Primary Neuronal Cultures

Open Access

Peer-reviewed

Research Article

Tessa M. Grabinski,

Affiliation Department of Translational Science and Molecular Medicine, College of Human Medicine, Michigan State University, Grand Rapids, MI, United States of America
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Andrew Kneynsberg,

Affiliation Department of Translational Science and Molecular Medicine, College of Human Medicine, Michigan State University, Grand Rapids, MI, United States of America
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Fredric P. Manfredsson,

Affiliation Department of Translational Science and Molecular Medicine, College of Human Medicine, Michigan State University, Grand Rapids, MI, United States of America
⨯
Nicholas M. Kanaan

* E-mail: Nicholas.kanaan@hc.msu.edu

Affiliation Department of Translational Science and Molecular Medicine, College of Human Medicine, Michigan State University, Grand Rapids, MI, United States of America
⨯

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Neurons
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Immunohistochemistry techniques
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Messenger RNA
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Neostriatum
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Signal amplification
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Peroxidases
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Phosphatases
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Gene expression
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