Claudin-2 Knockout by TALEN-Mediated Gene Targeting in MDCK Cells: Claudin-2 Independently Determines the Leaky Property of Tight Junctions in MDCK Cells

Open Access

Peer-reviewed

Research Article

Shinsaku Tokuda ,

* E-mail: furuse@nips.ac.jp

Affiliation Division of Cell Biology, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650–0017, Japan
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Mikio Furuse

Affiliations Division of Cell Biology, Department of Physiology and Cell Biology, Kobe University Graduate School of Medicine, Kobe 650–0017, Japan, Division of Cerebral Structure, National Institute for Physiological Sciences, Okazaki 444–8787, Japan, Department of Physiological Sciences, The Graduate University for Advanced Studies (SOKENDAI), Okazaki 444–8585, Japan
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Cloning
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TALENs
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Immunofluorescence
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Cell staining
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DNA cloning
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Transfection
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Polymerase chain reaction
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Permeability
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