Liquid-Based Iterative Recombineering Method Tolerant to Counter-Selection Escapes

• Masahiro Tominaga,

  Affiliation Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University, 1-33 Yayoi-Cyo, Inage-ku, Chiba 263-8522, Japan

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• Shigeko Kawai-Noma,

  Affiliation Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University, 1-33 Yayoi-Cyo, Inage-ku, Chiba 263-8522, Japan

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• Ikuro Kawagishi,

  Affiliation Department of Frontier Bioscience, Hosei University, 3-7-2, Koganei, Tokyo 184-8584, Japan

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• Yoshiyuki Sowa,

  Affiliations Department of Frontier Bioscience, Hosei University, 3-7-2, Koganei, Tokyo 184-8584, Japan, Research Center for Micro-Nano Technology, Hosei University, 3-11-15 Midori-cho, Tokyo 184-8584, Japan

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• Kyoichi Saito,

  Affiliation Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University, 1-33 Yayoi-Cyo, Inage-ku, Chiba 263-8522, Japan

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• Daisuke Umeno

  * E-mail: umeno@faculty.chiba-u.jp

  Affiliations Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Chiba University, 1-33 Yayoi-Cyo, Inage-ku, Chiba 263-8522, Japan, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

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